Developing Somatic Embryos
نویسندگان
چکیده
منابع مشابه
The effects of polyethylene glycol on gene expression of developing white spruce somatic embryos.
Somatic embryogenic cultures of white spruce (Picea glauca) represent a valuable system to study molecular mechanisms regulating embryo development because many embryos of defined developmental stages can be generated. The inclusion of polyethylene glycol (PEG) in the maturation medium can improve the number and quality of embryos produced. To learn more about the mechanism of action of PEG, we...
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BACKGROUND The nuclear mitotic apparatus (NuMA) plays a central role in the assembly and maintenance of spindle poles. Somatic cell nuclear transfer (SCNT) studies on non-human primates have shown that meiotic spindle removal during enucleation causes depletion of NuMA and the minus-end-directed motor protein (HSET) from the ooplasm, and this in turn leads to failure of embryo development. To d...
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Developing chick embryos are highly susceptible to infection with strains on V. cholerae representing Gardner and Venkatraman's 6 groups and the types Inaba and Ogawa. There is a moderate decrease in susceptibility with advancing age of the embryo. The influence of dosage on survival rates is not marked, probably because a minimal dose, consisting of a very few organisms, is sufficient to produ...
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Using the computer-assisted method of smoothed spatial averaging, spatial and temporal patterns of cell distribution and mitotic activity were analyzed in the cranial mesenchyme underlying the mesencephalic neural folds of mouse embryos maintained in roller tube culture. Total cell density increased in central and medial mesenchymal regions after 12 hr in culture, decreased after 18 hr, and sho...
متن کاملArginase is inoperative in developing soybean embryos.
Arginase (EC 3.5.3.1) transcript level and activity were measured in soybean (Glycine max L.) embryos from the reserve deposition stage to postgermination. Using a cDNA probe for a small soybean arginase gene family, no transcript was detected in developing embryos. However, arginase transcripts increased sharply on germination, reaching a maximum at 3 to 5 d after germination. There was low bu...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1992
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.4.4.367